{"id":29,"date":"2015-12-02T10:21:01","date_gmt":"2015-12-02T16:21:01","guid":{"rendered":"http:\/\/www.vanderbilt.edu\/cit\/?page_id=29"},"modified":"2018-02-26T11:23:07","modified_gmt":"2018-02-26T17:23:07","slug":"high-content-screening-microscopy","status":"publish","type":"page","link":"https:\/\/www.vanderbilt.edu\/cit\/high-content-screening-microscopy\/","title":{"rendered":"High-Content Screening Microscopy"},"content":{"rendered":"<div class=\"flex_column av_one_half  flex_column_div av-zero-column-padding first  avia-builder-el-0  el_before_av_one_half  avia-builder-el-first  \" style='border-radius:0px; '><section class=\"av_textblock_section \"  itemscope=\"itemscope\" itemtype=\"https:\/\/schema.org\/CreativeWork\" ><div class='avia_textblock  '   itemprop=\"text\" ><p>The Center for Innovative Technology (CIT) is making a <strong>Thermo Scientific ArrayScan XTI<\/strong>\u00a0<strong>Infinity Live<\/strong> high-content screening microscope available to the 91³Ô¹ÏÍø community. High-content analysis (HCA) technology allows users to collect data for many cellular features simultaneously across entire multiwell plates. In addition to a 7-color LED fluorescence illumination, this highly flexible, fully-automated high-content platform allows the user to choose between widefield and confocal imaging mode, brightfield and fluorescence illumination, and live or fixed-cell experimental setups.<\/p>\n<p>The powerful, highly intuitive software package enables online analysis of entire plates with pre-stablished and\u00a0fully customizable automated analysis workflows.<\/p>\n<p>New high-content screening clients\u00a0should submit a brief <a href=\"https:\/\/vanderbilt.corefacilities.org\/sc\/4369\/vanderbilt-center-for-innovative-technology-cit-core?tab=services\" target=\"_blank\" rel=\"noopener\">Project Request in iLab<\/a>. This will help us understand your analysis needs and provide you with a tailored approach. All users should also submit a <a href=\"https:\/\/vanderbilt.box.com\/cit-plate-layout\" target=\"_blank\" rel=\"noopener\">plate layout form<\/a> for every plate they would like to have read and analyzed.<\/p>\n<\/div><\/section><\/div><div class=\"flex_column av_one_half  flex_column_div av-zero-column-padding   avia-builder-el-2  el_after_av_one_half  el_before_av_button_big  avia-builder-el-last  \" style='border-radius:0px; '><div  class='avia-image-container avia_animated_image avia_animate_when_almost_visible fade-in av-styling-no-styling av-hover-grow   avia-builder-el-3  avia-builder-el-no-sibling  avia-align-center '  itemprop=\"image\" itemscope=\"itemscope\" itemtype=\"https:\/\/schema.org\/ImageObject\"  ><div class='avia-image-container-inner'><div class='avia-image-overlay-wrap'><img decoding=\"async\" class='wp-image-68 avia-img-lazy-loading-not-68 avia_image' src=\"https:\/\/cdn.vanderbilt.edu\/vu-wp0\/wp-content\/uploads\/sites\/166\/2015\/12\/27223125\/ArrayScan_main2.jpg\" alt='ArrayScan HCS Microscope in Nashville, TN' title='ArrayScan HCS Microscope in Nashville, TN' height=\"246\" width=\"425\"  itemprop=\"thumbnailUrl\" srcset=\"https:\/\/cdn.vanderbilt.edu\/vu-wp0\/wp-content\/uploads\/sites\/166\/2015\/12\/27223125\/ArrayScan_main2.jpg 425w, https:\/\/cdn.vanderbilt.edu\/vu-wp0\/wp-content\/uploads\/sites\/166\/2015\/12\/27223125\/ArrayScan_main2-300x174.jpg 300w\" sizes=\"(max-width: 425px) 100vw, 425px\" \/><\/div><\/div><\/div><\/div><\/p>\n<div  class='avia-button-wrap avia-button-center  avia-builder-el-4  el_after_av_one_half  el_before_av_one_half ' ><a href="https:\/\/www.vanderbilt.edu\/cit\/contact-us\/" class='avia-button avia-button-fullwidth   avia-icon_select-yes-left-icon avia-color-blue '  style='color:#ffffff; ' ><span class='avia_button_icon avia_button_icon_left' aria-hidden='true' data-av_icon='\ue805' data-av_iconfont='entypo-fontello'><\/span><span class='avia_iconbox_title' >Contact Us<\/span><span class='avia_button_background avia-button avia-button-fullwidth avia-color-custom' style='background-color:#0059c6;'><\/span><\/a><\/div>\n<div class=\"flex_column av_one_half  flex_column_div av-zero-column-padding first  avia-builder-el-5  el_after_av_button_big  el_before_av_one_half  avia-builder-el-first  \" style='border-radius:0px; '><p><div  style='padding-bottom:10px; ' class='av-special-heading av-special-heading-h3    avia-builder-el-6  el_before_av_table  avia-builder-el-first  '><h3 class='av-special-heading-tag '  itemprop=\"headline\"  >Hardware<\/h3><div class='special-heading-border'><div class='special-heading-inner-border' ><\/div><\/div><\/div><br \/>\n<div class='avia-data-table-wrap avia_responsive_table'><table  class='avia-table avia-data-table avia-table-1  avia-builder-el-7  el_after_av_heading  avia-builder-el-last  avia_pricing_default '  itemscope=\"itemscope\" itemtype=\"https:\/\/schema.org\/Table\" ><tbody><tr class=''><td class='avia-highlight-col'>Colors<\/td><td class=''>Seven-color, LED solid-state illumination:<br \/>\nreduces intensity fluctuations and optical component wear<br \/>\neliminates moving parts, and reduces both integration and channel switching times<br \/>\nBlue (386\/23 nm)<br \/>\nCyan (438\/24 nm)<br \/>\nGreen or Yellow (485\/20 nm)<br \/>\nRed (549\/15 nm)<br \/>\nScarlet (560\/25 nm)<br \/>\nFar-Red (650\/13 nm)<br \/>\nNear-Infrared (740\/13 nm)<br \/>\nPlus brightfield module for label-free cell detection<\/td><\/tr><tr class=''><td class='avia-highlight-col'>Emission Filters<\/td><td class=''>Flexibility w\/ 6-position emission filter wheel and 5-position dichroic mirror<br \/>\nBGRFRN (multiband): 412-460 nm, 503-531 nm, 590-624, 677-712 nm, 769-850 nm<br \/>\nRS (brighter Green\/Red-Scarlet for FRET): 573-658 nm use with BGRFN or BGS dichroic)<br \/>\nBGS (multiband): 412-460 nm, 503-531 nm, 580-650 nm<br \/>\nC (dedicated CFP): 467-498 nm (use w\/ C dichroic)<br \/>\nY (dedicated YFP): 527-560 nm (use w\/ Y dichroic)<br \/>\nN (dedicated Near-IR): 769-850 nm (use w\/ N dichroic)<\/td><\/tr><tr class=''><td class='avia-highlight-col'>Optics<\/td><td class=''>High Level of Sensitivity:<br \/>\nCrEST X-Light Spinning Disk Confocal Module (w\/Nipkow spinning disk)<br \/>\nAxio Z1 Observer components from Carl Zeiss&#x2122;<\/td><\/tr><tr class=''><td class='avia-highlight-col'>Objectives<\/td><td class=''>5x (0.25 NA) Zeiss Fluar (WD 12.5 mm)<br \/>\n10x (0.30 NA) Zeiss EC Plan NeoFluar (WD 5.2 mm)<br \/>\n10x (0.45 NA) Zeiss Plan Apochromat*<br \/>\n20x (0.40 NA) Zeiss LD Plan-Neofluar (WD 7.65 mm, correction collar)<br \/>\n20x (0.80 NA) Zeiss Plan Apochromat*<br \/>\n40x (0.75 NA) Zeiss Plan Neofluar*<br \/>\n*increased sensitivity, use these objectives with thin-bottom plates only <\/td><\/tr><tr class=''><td class='avia-highlight-col'>Camera<\/td><td class=''>Sony&#x2122; SuperHAD CCD camera:<br \/>\n14-bit dynamic range<br \/>\n2208&#215;2208 pixel array<br \/>\n4.54 micron pixel size to capture the max. information<\/td><\/tr><tr class=''><td class='avia-highlight-col'>Stage<\/td><td class=''>Automated stage with plate insert accepting SBS standard multiwell plates for imaging (8 wells, 6-well plate, 1536-well plate, 96-well plate, 24-well plate, 384-well plate). <\/td><\/tr><tr class=''><td class='avia-highlight-col'>Live Cell Imaging<\/td><td class=''>Live cell chamber hardware and environmental controller unit controlling temperature, CO2, and passive humidity control. <\/td><\/tr><\/tbody><\/table><\/div><style type='text\/css'><\/style><\/p><\/div><div class=\"flex_column av_one_half  flex_column_div av-zero-column-padding   avia-builder-el-8  el_after_av_one_half  el_before_av_one_half  \" style='border-radius:0px; '><p><div  style='padding-bottom:10px; ' class='av-special-heading av-special-heading-h3    avia-builder-el-9  el_before_av_table  avia-builder-el-first  '><h3 class='av-special-heading-tag '  itemprop=\"headline\"  >Software<\/h3><div class='special-heading-border'><div class='special-heading-inner-border' ><\/div><\/div><\/div><br \/>\n<div class='avia-data-table-wrap avia_responsive_table'><table  class='avia-table avia-data-table avia-table-2  avia-builder-el-10  el_after_av_heading  avia-builder-el-last  avia_pricing_default '  itemscope=\"itemscope\" itemtype=\"https:\/\/schema.org\/Table\" ><tbody><tr class=''><td class='avia-highlight-col'>HCS StudioTM Cell Analysis Software<\/td><td class=''>Open access to many image formats (TIF, DIB, BMP, JPEG, etc.)<br \/>\nAbility to compare and optimize two biological extents (positive and negative controls)<br \/>\nMeasurements at cell-, well-, and field-level.<br \/>\n30+ pre-established assays that can be optimized to your cells:<\/p>\n<p>Cell Cycle<\/p>\n<p>Cell Health<\/p>\n<p>Cell Motility<\/p>\n<p>Cell Spreading<\/p>\n<p>Colocalization<\/p>\n<p>Comets<\/p>\n<p>Compartmental Analysis<\/p>\n<p>Cytoplasm to Cell Membrane Translocation<\/p>\n<p>Cytoplasm to Nucleus Translocation<\/p>\n<p>Micronuclei<\/p>\n<p>Molecular Translocation<\/p>\n<p>Morphology Explorer<\/p>\n<p>Multiparameter Cytotoxicity<\/p>\n<p>Neuronal Profiling<\/p>\n<p>Organelle Health<\/p>\n<p>Spot Detector<\/p>\n<p>Target Activation<\/p>\n<p>Tube Formation<\/p>\n<p>Kinetics<\/p>\n<p>Zebrafish Toxicology<\/td><\/tr><\/tbody><\/table><\/div><style type='text\/css'><\/style><\/p><\/div><\/p>\n<div class=\"flex_column av_one_half  flex_column_div av-zero-column-padding first  avia-builder-el-11  el_after_av_one_half  el_before_av_one_half  column-top-margin\" style='border-radius:0px; '><div  class='avia-image-container avia_animated_image avia_animate_when_almost_visible left-to-right av-styling-    avia-builder-el-12  avia-builder-el-no-sibling  avia-align-center '  itemprop=\"image\" itemscope=\"itemscope\" itemtype=\"https:\/\/schema.org\/ImageObject\"  ><div class='avia-image-container-inner'><div class='avia-image-overlay-wrap'><img decoding=\"async\" class='wp-image-136 avia-img-lazy-loading-not-136 avia_image' src=\"https:\/\/cdn.vanderbilt.edu\/vu-wp0\/wp-content\/uploads\/sites\/166\/2015\/12\/27223014\/ArrayScan_bottom1.jpg\" alt='91³Ô¹ÏÍø Thermo ArrayScan Infinity XTI' title='91³Ô¹ÏÍø Thermo ArrayScan Infinity XTI' height=\"420\" width=\"500\"  itemprop=\"thumbnailUrl\" srcset=\"https:\/\/cdn.vanderbilt.edu\/vu-wp0\/wp-content\/uploads\/sites\/166\/2015\/12\/27223014\/ArrayScan_bottom1.jpg 500w, https:\/\/cdn.vanderbilt.edu\/vu-wp0\/wp-content\/uploads\/sites\/166\/2015\/12\/27223014\/ArrayScan_bottom1-300x252.jpg 300w, https:\/\/cdn.vanderbilt.edu\/vu-wp0\/wp-content\/uploads\/sites\/166\/2015\/12\/27223014\/ArrayScan_bottom1-450x378.jpg 450w\" sizes=\"(max-width: 500px) 100vw, 500px\" \/><\/div><\/div><\/div><\/div>\n<div class=\"flex_column av_one_half  flex_column_div av-zero-column-padding   avia-builder-el-13  el_after_av_one_half  el_before_av_hr  column-top-margin\" style='border-radius:0px; '><div  class='avia-image-container avia_animated_image avia_animate_when_almost_visible right-to-left av-styling-    avia-builder-el-14  avia-builder-el-no-sibling  avia-align-center '  itemprop=\"image\" itemscope=\"itemscope\" itemtype=\"https:\/\/schema.org\/ImageObject\"  ><div class='avia-image-container-inner'><div class='avia-image-overlay-wrap'><img decoding=\"async\" class='wp-image-138 avia-img-lazy-loading-not-138 avia_image' src=\"https:\/\/cdn.vanderbilt.edu\/vu-wp0\/wp-content\/uploads\/sites\/166\/2015\/12\/27223013\/ArrayScan_bottom2-1.jpg\" alt='Thermo ArrayScan Infinity - Environmental Chamber Setup' title='Thermo ArrayScan Infinity - Environmental Chamber Setup' height=\"420\" width=\"500\"  itemprop=\"thumbnailUrl\" srcset=\"https:\/\/cdn.vanderbilt.edu\/vu-wp0\/wp-content\/uploads\/sites\/166\/2015\/12\/27223013\/ArrayScan_bottom2-1.jpg 500w, https:\/\/cdn.vanderbilt.edu\/vu-wp0\/wp-content\/uploads\/sites\/166\/2015\/12\/27223013\/ArrayScan_bottom2-1-300x252.jpg 300w, https:\/\/cdn.vanderbilt.edu\/vu-wp0\/wp-content\/uploads\/sites\/166\/2015\/12\/27223013\/ArrayScan_bottom2-1-450x378.jpg 450w\" sizes=\"(max-width: 500px) 100vw, 500px\" \/><\/div><\/div><\/div><\/div>\n<div  style='height:50px' class='hr hr-invisible   avia-builder-el-15  el_after_av_one_half  el_before_av_one_full '><span class='hr-inner ' ><span class='hr-inner-style'><\/span><\/span><\/div>\n<div class=\"flex_column av_one_full  flex_column_div av-zero-column-padding first  avia-builder-el-16  el_after_av_hr  el_before_av_hr  \" style='border-radius:0px; '><div class='avia-data-table-wrap avia_responsive_table'><table  class='avia-table avia-data-table avia-table-3  avia-builder-el-17  avia-builder-el-no-sibling  avia_pricing_default '  itemscope=\"itemscope\" itemtype=\"https:\/\/schema.org\/Table\" ><caption>High-Content Screening Bioapplications Quick Overview<\/caption><tbody><tr class='avia-heading-row'><th class='avia-highlight-col'>Bioapplication Analysis<\/th><th class=''>Function<\/th><th class=''>Uses<\/th><th class=''>Applications<\/th><\/tr><tr class=''><td class='avia-highlight-col'>Cell Cycle<\/td><td class=''>Multiparameter assay that automatically classifies cell cycle phase based on DNA content, and correlates levels of up to three related targets at both the single cell and well population levels.<\/td><td class=''>Flexible application for screening of effectors of cell cycle progression, target validation, general life science research, and characterization and quality control of cell types.<\/td><td class=''>Cancer, Cell Signaling, Basic Research<\/td><\/tr><tr class=''><td class='avia-highlight-col'>Cell Health<\/td><td class=''>Enables examination of a variety of cell health markers including cell viability, cytotoxicity, and apoptosis. Applies advanced cell-level logic functions for discriminating between Early, Middle, or Late toxicity stages.<\/td><td class=''>Generate critical toxicology information to aid in the identification, selection, and prioritization of potential therapeutic candidates. Provide relevant, high content information for more reliable insight into the potential toxicity of drug candidates. Narrow field of drug candidates to undergo animal testing to the most positive compounds. Reveal mechanistic information critical to understanding toxic effects.<\/td><td class=''>Toxicology, Cell Survival, Cancer<\/td><\/tr><tr class=''><td class='avia-highlight-col'>Cell Motility<\/td><td class=''>Quantifies the area of the track produced by motile cells on a surface (2D) of fluorescent beads.<\/td><td class=''>Analysis of cellular motility in a fixed endpoint assay, as opposed to vector tracing of living cells. Also useful for profiling cancer cell lines by assessing compounds that inhibit motility or metastasis.<\/td><td class=''>Cancer, Cell Signaling, Inflammation, Wound Healing, Cellular Profiling, Angiogenesis, Vascular Disease, Other<\/td><\/tr><tr class=''><td class='avia-highlight-col'>Cell Spreading<\/td><td class=''>Measures shape and size of cells and colonies.<\/td><td class=''>Analysis of cellular shape change during attachement, motility, or differentiation. Successfully used to analyze differential adhesion properties on various matrices. Also allows the user to measure colony formation and nuclear count.<\/td><td class=''>Cancer, Cell Signaling, Cellular Profiling<\/td><\/tr><tr class=''><td class='avia-highlight-col'>Colocalization<\/td><td class=''>Enables the measurement within multiple cellular regions for the presence or coincidence of fluorescent targets.<\/td><td class=''>Quantifies the dregree of colocalization of a target of interest (often a protein) with other proteins, organelles, subcellular compartments.<\/td><td class=''>Protein-protein interactions, Internalization, Intracellular Trafficking, Cytoskeletal Protein Formation\/Disruption<\/td><\/tr><tr class=''><td class='avia-highlight-col'>Comets<\/td><td class=''>Enables detection of genotoxicity and DNA damage by the automated quantitation of comets (electrophoresed nuclei). Measured features include the %DNA in Tail and Olive Tail Movement for determining DNA damage potential of compounds. Can be combined with FISH analysis using other stains in additional channels.<\/td><td class=''>Screen compounds for genotoxicity earlier in the drug discovery process; get better information during lead optimization and SAR studies.<\/td><td class=''>Cytotoxicity, Genotoxicity, Toxicity Profiling, Cancer<\/td><\/tr><tr class=''><td class='avia-highlight-col'>Compartmental Analysis<\/td><td class=''>General purpose application with a high level of flexibility for analysis of a wide range of biological assays.<\/td><td class=''>Intensity analysis including ratiometric intensity analysis, translocation analysis, and detection of discrete cellular spots and objects in different compartments.<\/td><td class=''>Pathway analysis, Cellular Signaling, Proliferation and Cell Death (PCD), Endocrine Profiling, Drug-Induced Liver Injury (DILI), DNA Damage, Others<\/td><\/tr><tr class=''><td class='avia-highlight-col'>Cytoplasm to Cell Membrane Translocation<\/td><td class=''>Specifically measures target translocation between cytoplasm and cell membrane.<\/td><td class=''>Unique application for analysis of targets such as PKC, GLUT-4, and ras. Also useful for comparison of receptor ratio between the cell surface interior.<\/td><td class=''>Cancer, Cell Signaling, Metabolic Disease, Other<\/td><\/tr><tr class=''><td class='avia-highlight-col'>Cytoplasm to Nucleus Translocation<\/td><td class=''>Patented method to monitor target translocation between two sub-cellular regions.<\/td><td class=''>Analysis of nuclear translocation for kinases and transcription factors, simple intensity measurements within the cell, determination of transfection efficiency, and analysis of nuclear or cytoplasmic protein levels.<\/td><td class=''>Cancer, Cardiovascular, Inflammation, Neurobiology, Cell Signaling, Infectious Disease, Others<\/td><\/tr><tr class=''><td class='avia-highlight-col'>Micronucleus<\/td><td class=''>Enables detection of genotoxicity through examining the frequency of micronucleus formation in single cells. Also reports early cytotoxicity (viability, cell cycle delay through %cytostasis), frequency of mononucleate and late-stage toxicity (permeabilized cells).<\/td><td class=''>Screen compounds for genotoxicity earlier in the drug discovery process; get better information during lead optimization and SAR studies.<\/td><td class=''>Cytotoxicity, Genotoxicity, Toxicity Profiling, Cancer <\/td><\/tr><tr class=''><td class='avia-highlight-col'>Molecular Translocation<\/td><td class=''>Multiparameter assay to measure and correlate multiple intracellular translocation events at the single cell level.<\/td><td class=''>Pathway analysis, kinase specific studies, transcription factor activity profiling.<\/td><td class=''>Multiple Target Translocation, Others<\/td><\/tr><tr class=''><td class='avia-highlight-col'>Morphology Explorer<\/td><td class=''>Detects and quantifies multi-cellular, cellular, sub-cellular morphological changes. Characterizes and measures cell proximity, cell colonies, process outgrowths, distribution and organization of intracellular fluorescent structures such as cytoskeletal fibers and discrete objects such as organelles. Does 1st order (intensity dependent) and 2nd order (intensity independent) texture analysis of intracellular stains.<\/td><td class=''>Includes analysis of colonies, cytoskeletal rearrangement, tube formation, intracellular protein distribution, cell shape and size changes, and process outgrowth.<\/td><td class=''>Intracellular Morphology, Whole Cell Morphology, Inter\/Multi-Cellular Morphology, Myoblast Formation, Cytoskeletal Rearrangement, Colony Formation, Tubes, Multi-Nucleated Cells, Functional Genomics, Cancer, Phenotypic Characterization, Neuroscience<\/td><\/tr><tr class=''><td class='avia-highlight-col'>Multiparameter Cytotoxicity<\/td><td class=''>Measures multiple cytotoxicity-related intensity and shape-based outputs in a single assay: nuclear morphology, cell permeability, cell count and either lysosomal pH or mitochondrial transmembrane potential.<\/td><td class=''>Profiling multiple aspects of complex cytotoxicity and apoptosis pathways. Monitoring multiple aspects of cell life\/death.<\/td><td class=''>Cytotoxicity Profiling, Cell Survival, Cancer, Nuclear Size\/Morphology Changes, Cell Membrane Permeability, Organelle Function<\/td><\/tr><tr class=''><td class='avia-highlight-col'>Neuronal Profiling<\/td><td class=''>Enables automated quantitation and correlation of neuronal and neurite morphology along with synaptogenesis. Analysis can range from simple neurite outgrowth analysis to complexity analysis of extended neurite outgrowth and branching, to correlative studies on synaptic function, determined by the colocalization of pre-and post-synaptic markers, all within one software package.<\/td><td class=''>A non-biased, automated, and validated method to investigate drug therapies for modulating neuronal growth and\/or neurotransmission. <\/td><td class=''>Neurite Detection, Synaptogenesis, Neurotoxicity Profiling, Paralysis, Alzheimer&#8217;s and Neurodegenerative Diseases, Depression, Epilepsy, Developmental Neurotoxicity<\/td><\/tr><tr class=''><td class='avia-highlight-col'>Organelle Health<\/td><td class=''>Enables examination of a variety of cell health markers including cell viability, cytotoxicity, and apoptosis. Applies advanced cell-level logic functions for discriminating between Early, Middle, or Late toxicity stages.<\/td><td class=''>Generate critical toxicology information to aid in the identification, selection, and prioritization of potential therapeutic candidates. Narrow field of drug candidates to undergo animal testing to the most promising compounds. Reveal mechanistic information critical to understanding toxic effects.<\/td><td class=''>Toxicology, Cell Survival, Cancer<\/td><\/tr><tr class=''><td class='avia-highlight-col'>Spot Detector<\/td><td class=''>Rapid yet flexible assay that measures &#8220;spots&#8221; representing internalized receptors or other discrete punctate targets in up to four channels.<\/td><td class=''><\/td><td class=''>Receptor Internalization, Beta-Arrestin Redistribution, Organelle Analysis (Lysosome, endosome, mitochondria, Golgi), Lipid Accumulation, Phospholipidosis, Cell Counting, Proliferation, Rare Event Analysis, Cell Viability, Mitotic- and S-phase Indexing, Cancer, Cell Signaling, GPCRs, Metabolic Disease, Others<\/td><\/tr><tr class=''><td class='avia-highlight-col'>Target Activation<\/td><td class=''>General purpose assay for measurement of average and total intensity within a user-defined cellular domain in each of 6 channels.<\/td><td class=''>Incl. rare event analysis, population analysis, gene expression\/knockdown, expression profiling.<\/td><td class=''>Cell Viability, Mitotic Index, Others<\/td><\/tr><tr class=''><td class='avia-highlight-col'>Tube Formation<\/td><td class=''>Measures degree of angiogenic tube formation by endothelial cells. Reports tube areas and lengths, node count, angiogenic index.<\/td><td class=''>Analysis of conditions or factors that promote or inhibit angiogenesis. Screening for therapeutic compounds.<\/td><td class=''>Cancer, Cell Signaling, Angiogenesis, Differentiation, Toxicity<\/td><\/tr><tr class=''><td class='avia-highlight-col'>Kinectics<\/td><td class=''>Versatile time-lapse assay to measure 2D displacement, distance travelled, length, speed, directionality, angle change, turn strength, spatial persistence, acceleration, net displacement, total distance, drift speed, temporal persistence.<\/td><td class=''>Kinetic measurements of any cell\/well feature vs. time curve. Motility measurements of position vs. time trace for each cell. Statistical summary of cell motility on a per-well level.<\/td><td class=''>Chemotaxis, Haptotaxis, Cancer, Wound Healing, Cell Dispersion, Invasion, Motility<\/td><\/tr><tr class=''><td class='avia-highlight-col'>Zebrafish Toxicology<\/td><td class=''>Quantifies morphological changes in 1-5 day Zebrafish embryos due to toxic stimuli. Images can be acquired in either brightfield or fluorescence. Additional fluorescence channels are used to measure spot formation and other biomarkers.<\/td><td class=''>Automated small animal toxicity screen to supplement in vitro cell-based toxicity studies.<\/td><td class=''>Zebrafish, Cytotoxicity, Genotoxicity, Toxicity Profiling, Development<\/td><\/tr><\/tbody><\/table><\/div><style type='text\/css'>.avia-table-3 td:nth-of-type(1):before { content: 'Bioapplication Analysis'; } .avia-table-3 td:nth-of-type(2):before { content: 'Function'; } .avia-table-3 td:nth-of-type(3):before { content: 'Uses'; } .avia-table-3 td:nth-of-type(4):before { content: 'Applications'; } <\/style><\/div><div  style='height:50px' class='hr hr-invisible   avia-builder-el-18  el_after_av_one_full  el_before_av_social_share '><span class='hr-inner ' ><span class='hr-inner-style'><\/span><\/span><\/div><\/p>\n<div  class='av-social-sharing-box  avia-builder-el-19  el_after_av_hr  avia-builder-el-last  '><div class='av-share-box'><h5 class='av-share-link-description av-no-toc '>Share this entry<\/h5><ul class='av-share-box-list noLightbox'><li class='av-share-link av-social-link-facebook' ><a target=\"_blank\" aria-label=\"Share on Facebook\" href="https:\/\/www.facebook.com\/sharer.php?u=https:\/\/www.vanderbilt.edu\/cit\/high-content-screening-microscopy\/&#038;t=High-Content%20Screening%20Microscopy" aria-hidden='false' data-av_icon='\ue8f3' data-av_iconfont='entypo-fontello' title='' data-avia-related-tooltip='Share on Facebook' rel=\"noopener\"><span class='avia_hidden_link_text'>Share on Facebook<\/span><\/a><\/li><li class='av-share-link av-social-link-twitter' ><a target=\"_blank\" aria-label=\"Share on Twitter\" href="https:\/\/twitter.com\/share?text=High-Content%20Screening%20Microscopy&#038;url=https:\/\/www.vanderbilt.edu\/cit\/?p=29" aria-hidden='false' data-av_icon='\ue8f1' data-av_iconfont='entypo-fontello' title='' data-avia-related-tooltip='Share on Twitter' rel=\"noopener\"><span class='avia_hidden_link_text'>Share on Twitter<\/span><\/a><\/li><li class='av-share-link av-social-link-linkedin' ><a target=\"_blank\" aria-label=\"Share on LinkedIn\" href="https:\/\/linkedin.com\/shareArticle?mini=true&#038;title=High-Content%20Screening%20Microscopy&#038;url=https:\/\/www.vanderbilt.edu\/cit\/high-content-screening-microscopy\/" aria-hidden='false' data-av_icon='\ue8fc' data-av_iconfont='entypo-fontello' title='' data-avia-related-tooltip='Share on LinkedIn' rel=\"noopener\"><span class='avia_hidden_link_text'>Share on LinkedIn<\/span><\/a><\/li><li class='av-share-link av-social-link-reddit' ><a target=\"_blank\" aria-label=\"Share on Reddit\" href="https:\/\/reddit.com\/submit?url=https:\/\/www.vanderbilt.edu\/cit\/high-content-screening-microscopy\/&#038;title=High-Content%20Screening%20Microscopy" aria-hidden='false' data-av_icon='\ue927' data-av_iconfont='entypo-fontello' title='' data-avia-related-tooltip='Share on Reddit' rel=\"noopener\"><span class='avia_hidden_link_text'>Share on Reddit<\/span><\/a><\/li><li class='av-share-link av-social-link-mail' ><a  aria-label=\"Share by Mail\" href="mailto:?subject=High-Content%20Screening%20Microscopy&#038;body=https:\/\/www.vanderbilt.edu\/cit\/high-content-screening-microscopy\/" aria-hidden='false' data-av_icon='\ue805' data-av_iconfont='entypo-fontello' title='' data-avia-related-tooltip='Share by Mail'><span class='avia_hidden_link_text'>Share by Mail<\/span><\/a><\/li><\/ul><\/div><\/div>\n","protected":false},"excerpt":{"rendered":"","protected":false},"author":959,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":[],"_links":{"self":[{"href":"https:\/\/www.vanderbilt.edu\/cit\/wp-json\/wp\/v2\/pages\/29"}],"collection":[{"href":"https:\/\/www.vanderbilt.edu\/cit\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.vanderbilt.edu\/cit\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.vanderbilt.edu\/cit\/wp-json\/wp\/v2\/users\/959"}],"replies":[{"embeddable":true,"href":"https:\/\/www.vanderbilt.edu\/cit\/wp-json\/wp\/v2\/comments?post=29"}],"version-history":[{"count":19,"href":"https:\/\/www.vanderbilt.edu\/cit\/wp-json\/wp\/v2\/pages\/29\/revisions"}],"predecessor-version":[{"id":1607,"href":"https:\/\/www.vanderbilt.edu\/cit\/wp-json\/wp\/v2\/pages\/29\/revisions\/1607"}],"wp:attachment":[{"href":"https:\/\/www.vanderbilt.edu\/cit\/wp-json\/wp\/v2\/media?parent=29"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}